Journal: PLOS Pathogens
Article Title: Globodera pallida virulence on major potato resistance has a common genetic basis across Western Europe
doi: 10.1371/journal.ppat.1014201
Figure Lengend Snippet: A The alternative allele frequency (AAF) of SNP T173N in 13 G. pallida populations as measured with allele-specific (AS) primers in a qPCR against the AAF according to DNA sequencing. B The validation of the AS-qPCR assay at four different labs. AAFs as determined by AS-qPCR by one lab are plotted against the AAFs as determined by qPCR by the other lab or against the AAF as determined by sequencing. Statistics in A and B are determined by linear regression models. Black dashed lines indicates y = x for visual support. C The AAFs of unselected and selected G. pallida populations as determined by qPCR in four different labs. Unselected populations were propagated on the susceptible variety Desiree and selected populations were propagated for four rounds on the GpaV vrn -containing variety Seresta. Statistics are based on one-sided t-tests.
Article Snippet: From each population, DNA was extracted from 60 cysts using the MasterPure Complete DNA & RNA Purification Kit (Lucigen, USA) following the manufacturers protocol.
Techniques: DNA Sequencing, Biomarker Discovery, Sequencing